DNA Purification BasicsThis guide provides a comprehensive introduction to DNA and RNA purification methods, including the basics of DNA isolation, plasmid growth and nucleic acid quantification.In...

Poor primer PCR design is the one major cause of all failures or false positives or negatives in single and multiplex PCR. Primers may fail for many different reasons and the major factors that aff...

In conventional PCR, the amplified DNA product, or amplicon, is detected in an end-point analysis. In real-time PCR, the accumulation of amplification product is measured as the reaction progresses...

Why is RNA extraction tougher than DNA extractionThe main reason is that RNA is less stable and easier to degrade compared to DNA. There are three main causes for RNA degradation:RNA is more reacti...