Immunoassays are used to measure the presence and/or concentration of an analyte (protein, hormone, antibody). A main characteristic of immunoassays is their high specificity, resulting from the use of analyte specific antibodies. These can detect the analyte of interest at even low concentration.
Depending on the format, immunoassays use different detection signals to indicate the binding of the antibody to the analyte, each with its specific advantages. Streamline data interpretation is achieved with automation and rapid measurement of detection signals. Immunodetection Enzyme-Linked Immunosorbent Assays (ELISA) are well-established single-analyte assays that delivers robust and reliable data. With a high-throughput capacity, they provide quick and efficient monitoring of critical biomarkers. Immunohistochemistry (IHC) is a method to detect antigen or hapten in tissue section cells by using the specific combination of antibody and antigen in biological tissue. Immunofluorescence (IF) is a technique that permits visualization of virtually many components in any given tissue or cell type. This broad capability is achieved through combinations of specific antibodies tagged with fluorophores. Western blot (WB) is often used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. Reagents/Buffers Immunohistochemistry (IHC) is a method to detect antigen or hapten in tissue section cells by using the specific combination of antibody and antigen in biological tissue. Immunofluorescence (IF) is a technique that permits visualization of virtually many components in any given tissue or cell type. This broad capability is achieved through combinations of specific antibodies tagged with fluorophores. RIPA lysate Loading Buffer Electrophoresis Buffer PBS/TBS Buffer Blocking Solution Antibody Diluent Transmembrane Buffer Stainning Buffer Decolorizing Solution |