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Recombinant protein is a manipulated form of protein produced through recombinant DNA technology. By inserting the DNA encoding the protein into bacterial or mammalian cells, we can get quantities of target proteins after amplifying expression and purification. Beyoscience offers a diverse selection of more than 4000 proteins for your research, including cytokines, growth factors, immune checkpoint proteins, CD antigen, FC Receptor, enzymes, hormone, etc.  Recombinant Proteins To make recombinant proteins, the gene is isolated and cloned into an expression vector. Generating a recombinant protein requires the protein expression system, protein purification system and protein identification systems. How to Make Recombinant Protein Basic steps to get recombinant Protein: 1. Amplification of gene of interest. 2. Insert into cloning vector. 3. Sub cloning into expression vector. 4. Transformation into protein expressing host (bacteria, yeast, mammalian cells or baculovirus-insect cell system). 5. Test for identification of recombinant protein.( Western blot or Fluoroscence) 6. Large scale production. (Large scale fermentor) 7. Isolation and purification. High-quality High Purity(>95%), Lower Endotoxin(<1.0EU/μL) Activity Validation Features & Advantages of Recombinant Proteins Strict Quality Control Standardized Operation Procedures, GMP-level Workshop Multiple Expression Systems E.coli, Yeast, Mammalian Cells, Insect Cells(baculovirus) Techinical Support Complete Pre-sales and After-sales Support Applications of Recombinant Proteins Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Heavy labeled protein as SRM/MRM standards Protein Expression System Comparison |